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Vectors lambda 200g and lambda 200c: two useful derivatives of lambda 2001.

C G Koh·L Barnett·S Brenner
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Abstract

We describe the construction and uses of two new phage lambda replacement vectors. Both are modifications of lambda 2001 which allows selection of cloned inserts by the Spi- phenotype, following the replacement of a gam+ filler fragment. Vector lambda 200g has a second gam gene with an amber mutation in its right arm which enables the selection of clones with inserts in the normal way in Su0 host bacteria and the maintenance of their genetic stability by subsequent growth in Su+ strains. Vector lambda 200c is designed for complementation studies. It contains a cat gene and the attP site of lambda. Recombinants can be inserted into the bacterial chromosome or into an episome with the aid of appropriate helpers.

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Attachment Sites, MicrobiologicalBacteriophage lambdaChloramphenicol O-AcetyltransferaseCloning, MolecularDNA Restriction EnzymesDNA, RecombinantGenes, SuppressorGenes, ViralGenetic Complementation TestGenetic VectorsMutationSuppression, GeneticJournal Article

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C G Koh, L Barnett, S Brenner. 1993-08-16. Vectors lambda 200g and lambda 200c: two useful derivatives of lambda 2001.. https://doi.org/10.1016/0378-1119(93)90353-5

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