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PubMed · 8394087

Peripheral benzodiazepine receptor modulation with phagocyte differentiation.

X Canat·A Guillaumont·M Bouaboula·C Poinot-Chazel·J M Derocq·P Carayon·G LeFur·P Casellas
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Abstract

Peripheral benzodiazepine receptor (PBR) was found to be less expressed in the immature phagocytic HL-60 and U-937 cell lines than in the more mature monocytic THP-1 cell line. Cell differentiation by several agents induced a strong enhancement of PBR density on these three phagocytic cell lines but not on the lymphocytic CEM cell line. Detailed analysis of phorbol 12-myristate 13-acetate-treated THP-1 cells showed an increased PBR expression and the rise came along with an increase of CD11a and CD11b antigens and a secretion of macrophagic cytokines tumor necrosis factor-alpha (TNF-alpha), interleukin (IL)-1 beta and IL-8. Quantitation of mRNA using polymerase chain reaction (PCR)-based technique showed that overexpression of PBR did not parallel mRNA expression, indicating a gene-independent regulation. These results suggest that PBR predominance on phagocytic cells could be related to maturation process.

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Antigens, CDBinding SitesCell DifferentiationCell LineDimethyl SulfoxideHumansInterferon-gammaInterleukin-1Interleukin-8PhagocytesRNA, MessengerReceptors, GABA-ATetradecanoylphorbol AcetateTumor Necrosis Factor-alphaComparative StudyJournal Article

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X Canat, A Guillaumont, M Bouaboula, C Poinot-Chazel, J M Derocq, P Carayon, G LeFur, P Casellas. 1993-08-03. Peripheral benzodiazepine receptor modulation with phagocyte differentiation.. https://doi.org/10.1016/0006-2952(93)90535-5

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