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PubMed · 8396934

IL-2 induces transient and specific decrease in cytosolic protein phosphatase PP1 activity in murine T cell lines.

S Matsuzawa·A Matsuda·Y Mizuno·K Kikuchi
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Abstract

In the IL-2-dependent murine cytotoxic T cell line CTLL-2, IL-2 induced a rapid and transient decrease in Co(2+)-trypsin-treated activity of protein phosphatase PP1. The PP1 activity declined to a minimum level, being 70% of control value, in 20 min after the addition of IL-2 but recovered to the control level within 45 min. The decrease of PP1 activity was dependent on IL-2 concentration and occurred specifically in cytosolic fraction. Similar alteration was observed in IL-2 sensitive murine T-lymphoblasts. Neither activity of protein phosphatase PP2A nor PP2C showed alteration during the IL-2 stimulation. These results suggest that PP1 plays an important role in early events of the intracellular growth signaling from the IL-2 receptor.

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AnimalsCell DivisionCell LineCytosolHumansInterleukin-2IsoenzymesKineticsLymphocyte ActivationMiceMice, Inbred C57BLPhosphoprotein PhosphatasesRecombinant ProteinsT-Lymphocytes, CytotoxicTime FactorsTrypsinJournal ArticleResearch Support, Non-U.S. Gov't

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S Matsuzawa, A Matsuda, Y Mizuno, K Kikuchi. 1993-09-15. IL-2 induces transient and specific decrease in cytosolic protein phosphatase PP1 activity in murine T cell lines.. https://doi.org/10.1006/bbrc.1993.2142

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