Bovine microsatellites: racial differences and association with SINE-elements.

S L Varvio; J Kaukinen

doi:10.1007/978-3-0348-8583-6_44

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Bovine microsatellites: racial differences and association with SINE-elements.

S L Varvio·J Kaukinen

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Abstract

Patterns of polymorphism at eight microsatellite loci in three cattle races are described: two large commercial breeds and one endangered landrace. Significant interracial allele frequency differences were found at six loci. The mean heterozygosity was slightly higher in the landrace (H = 0.75) than in the others (H = 0.69). The difference is smaller than that found by DNA-fingerprinting. Intrapopulation distributions of microsatellite allele size (dinucleotide repeat number) were generally bimodal, with certain intermediate repeat types lacking. Sequencing of individual alleles revealed some hidden heterogeneity: an allele defined by PCR-product size actually corresponded to different sequence motifs in different races. Two of the microsatellites occurred as tails of SINE-elements. In contrast to some earlier reports, the position of one of the amplification primers within a high-copy-number SINE-element did not disturb microsatellite amplification; even multiplex PCR was possible.

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Alleles Animals Base Sequence Cattle DNA Fingerprinting DNA, Satellite Female Gene Frequency Male Molecular Sequence Data Polymorphism, Genetic Repetitive Sequences, Nucleic Acid Species Specificity Comparative Study Journal Article Research Support, Non-U.S. Gov't

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S L Varvio, J Kaukinen. 1993. Bovine microsatellites: racial differences and association with SINE-elements.. https://doi.org/10.1007/978-3-0348-8583-6_44

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