cDNA libraries from human tissues and cell lines.

A Swaroop; J Xu

doi:10.1159/000133595

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cDNA libraries from human tissues and cell lines.

A Swaroop·J Xu

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Abstract

We report the construction of 34 cDNA libraries from human tissues and cell lines in lambda phage vectors (Charon BS, lambda gt11, lambda SHK, or lambda zapII). The cDNA was synthesized from poly A+ RNA, using oligo-dT as a primer, and size-selected before ligation to vector arms. High-complexity cDNA libraries from human tissues and cell lines should be a valuable resource for genome mapping studies and identification of disease genes.

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Bacteriophage lambda Base Sequence Cell Line Culture Techniques DNA, Complementary Genomic Library Humans Molecular Sequence Data Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

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A Swaroop, J Xu. 1993. cDNA libraries from human tissues and cell lines.. https://doi.org/10.1159/000133595

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