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PubMed · 8406046

Improved bacterial hosts for regulated expression of genes from lambda pL plasmid vectors.

T A Patterson·N Costantino·S Dasgupta·D L Court
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Abstract

The construction and use of a set of Escherichia coli strains with defective lambda prophages that facilitate expression of genes cloned in lambda pL-plasmid vectors is described. These bacteria allow high and regulated expression of such genes, whereas a kanamycin-resistance marker (KmR) on the prophage allows easy identification and genetic transfer from strain to strain. Optimal conditions for examining gene expression with the pL-vector systems using these strains are discussed.

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Bacteriophage lambdaCloning, MolecularDefective VirusesEscherichia coliGene Expression Regulation, BacterialGenes, ViralGenetic VectorsPlasmidsJournal ArticleResearch Support, U.S. Gov't, P.H.S.

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T A Patterson, N Costantino, S Dasgupta, D L Court. 1993-09-30. Improved bacterial hosts for regulated expression of genes from lambda pL plasmid vectors.. https://doi.org/10.1016/0378-1119(93)90517-7

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