Multiple Ca2+ channel types coexist to regulate synaptosomal neurotransmitter release.

T J Turner; M E Adams; K Dunlap

doi:10.1073/pnas.90.20.9518

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Multiple Ca2+ channel types coexist to regulate synaptosomal neurotransmitter release.

T J Turner·M E Adams·K Dunlap

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Abstract

The regulation of excitation-secretion coupling by Ca2+ channels is a fundamental property of the nerve terminal. Peptide toxins that block specific Ca2+ channel types have been used to identify which channels participate in neurotransmitter release. Subsecond measurements of [3H]-glutamate and [3H]dopamine release from rat striatal synaptosomes showed that P-type channels, which are sensitive to the Agelenopsis aperta venom peptide omega-Aga-IVA, trigger the release of both transmitters. Dopamine (but not glutamate) release was also controlled by N-type, omega-conotoxin-sensitive channels. With strong depolarizations, where neither toxin was very effective alone, a combination of omega-Aga-IVA and omega-conotoxin produced a synergistic inhibition of 60-80% of Ca(2+)-dependent dopamine release. The results suggest that multiple Ca2+ channel types coexist to regulate neurosecretion under normal physiological conditions in the majority of nerve terminals. P- and N-type channels coexist in dopaminergic terminals, while P-type and a omega-conotoxin- and omega-Aga-IVA-resistant channel coexist in glutamatergic terminals. Such an arrangement could lend a high degree of flexibility in the regulation of transmitter release under diverse conditions of stimulation and modulation.

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Animals Calcium Calcium Channel Blockers Calcium Channels Corpus Striatum Dopamine Glutamates In Vitro Techniques Mollusk Venoms Peptides Rats Spider Venoms Synaptic Transmission Synaptosomes omega-Agatoxin IVA omega-Conotoxin GVIA Journal Article Research Support, U.S. Gov't, P.H.S.

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T J Turner, M E Adams, K Dunlap. 1993-10-15. Multiple Ca2+ channel types coexist to regulate synaptosomal neurotransmitter release.. https://doi.org/10.1073/pnas.90.20.9518

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