A simplified method for generating step-wise deletions using PCR.

M Kitabatake; H Inokuchi

doi:10.1016/0378-1119(93)90539-f

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A simplified method for generating step-wise deletions using PCR.

M Kitabatake·H Inokuchi

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Abstract

A simple and general method is described for the generation of ordered deletions for DNA sequencing. Nicked plasmids, the substrates for step-wise digestion by exonuclease III, are obtained after the self-ligation of PCR products with phosphorylated and non-phosphorylated primers and plasmid DNAs as template. The method is suitable for use with any plasmid vector and for generation of deletion clones with deletions in both possible directions.

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Base Sequence Cloning, Molecular DNA, Single-Stranded Molecular Sequence Data Oligodeoxyribonucleotides Phosphorylation Plasmids Polymerase Chain Reaction Sequence Analysis, DNA Sequence Deletion Journal Article Research Support, Non-U.S. Gov't

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M Kitabatake, H Inokuchi. 1993-01-15. A simplified method for generating step-wise deletions using PCR.. https://doi.org/10.1016/0378-1119(93)90539-f

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