Capture assay for specific IgE. An improved quantitative method.

V Olivieri; I Beccarini; G Gallucci; T Romano; F Santoro

doi:10.1016/0022-1759(93)90071-e

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Capture assay for specific IgE. An improved quantitative method.

V Olivieri·I Beccarini·G Gallucci·T Romano·F Santoro

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Abstract

A capture assay for the measurement of specific IgE in the serum of allergic patients is described, using monoclonal anti-human IgE (coated to the wells of a microtiter plate) and biotinylated allergens in solution. In a single incubation, IgE is bound to the solid phase through the Fc fragment and biotinylated allergens react with their specific IgE Fab regions, if present. In a second step, streptavidin-HRP conjugate is added to reveal the amount of biotin fixed on the solid phase. Quantitative determinations are obtained by comparison with a standard curve of total IgE incubated with a biotinylated monoclonal anti-IgE, complementary to the one employed as capture antibody. The assay is unaffected by allergen-specific IgG, shows good intra- and interassay reproducibility and is linear over a wide range of specific IgE concentrations. The method could be used with a wide range of different allergens.

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Animals Antibody Specificity Humans Hypersensitivity Immunoglobulin E Immunoglobulin G Mice Mice, Inbred BALB C Radioallergosorbent Test Reproducibility of Results Journal Article

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V Olivieri, I Beccarini, G Gallucci, T Romano, F Santoro. 1993-01-04. Capture assay for specific IgE. An improved quantitative method.. https://doi.org/10.1016/0022-1759(93)90071-e

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