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PubMed · 8432979

Recombinase activating gene expression in thymic subpopulations. A transitional cell type has lost RAG-2 but not RAG-1.

J J Campbell·Y Hashimoto
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Abstract

We have used a highly sensitive Northern blot probing technique to examine RAG-1 and RAG-2 mRNA expression in unmanipulated murine thymocytes of various developmental phenotypes sorted by flow cytometry. We have found that neither RAG-1 nor RAG-2 are down-regulated until thymocytes reach the TCRhi stage. TCRhi, CD8+CD4+ cells have lost RAG-2 mRNA but continue to express significant levels of RAG-1 mRNA, providing molecular evidence that this cell type is transitional between the TCRlo, CD4+CD8+ and the TCRhi, CD4+CD8-, or CD4-CD8+ phenotypes.

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AnimalsBase SequenceDNA-Binding ProteinsFlow CytometryGene ExpressionGene Rearrangement, T-LymphocyteHomeodomain ProteinsMiceMice, Inbred C57BLMolecular Sequence DataOligodeoxyribonucleotidesProteinsRNA, MessengerReceptors, Antigen, T-CellRecombination, GeneticThymus Glandbeta 2-MicroglobulinJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, P.H.S.

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J J Campbell, Y Hashimoto. 1993-02-15. Recombinase activating gene expression in thymic subpopulations. A transitional cell type has lost RAG-2 but not RAG-1.. https://pubmed.ncbi.nlm.nih.gov/8432979/

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