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Error analysis in manual and automated DNA sequencing.

F Khurshid·S Beck
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Abstract

The frequency, position, and type of errors were analyzed for sequence data which were generated with the help of (I) a manual digitizer, (II) an off-line automated film reader, and (III) an on-line automated gel reader. About 400 film/gel readings were analyzed and the results were used to generate error profiles for each method. The profiles identify several method- and project-specific problem areas and provide useful guidance for the assessment of DNA sequence data in general.

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Evaluation Studies as TopicHumansReproducibility of ResultsSequence Analysis, DNAComparative StudyJournal Article

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F Khurshid, S Beck. 1993. Error analysis in manual and automated DNA sequencing.. https://doi.org/10.1006/abio.1993.1019

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