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Biodegradable microparticles for oral immunization.

D T O'Hagan·J P McGee·J Holmgren·A M Mowat·A M Donachie·K H Mills·W Gaisford·D Rahman·S J Challacombe
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Abstract

Ovalbumin (OVA) was entrapped in poly(lactide-co-glycolide) microparticles and administered to mice. Following intraperitoneal immunization, the microparticles induced both proliferative T-cell responses and cytotoxic T-cell responses in spleen cells. Following oral immunization, the mean salivary IgA antibody response to microparticles was significantly greater than the response to soluble OVA (p < 0.0001). Serum IgG antibody levels were also significantly greater in the group administered microparticles (p < 0.001). Cholera toxin B subunit was also entrapped in microparticles. Following oral immunization in mice, specific antibody-secreting cells were detected both in the spleens and in the mesenteric lymph nodes.

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Administration, OralAnimalsAntibodies, ViralBiodegradation, EnvironmentalCholera ToxinDelayed-Action PreparationsDrug CompoundingEmulsionsImmunoglobulin A, SecretoryImmunoglobulin GInjections, IntraperitonealLymph NodesMaleMiceMice, Inbred BALB CMicrospheresOvalbuminPolyglactin 910SpleenT-Lymphocyte SubsetsJournal ArticleResearch Support, Non-U.S. Gov't

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D T O'Hagan, J P McGee, J Holmgren, A M Mowat, A M Donachie, K H Mills, W Gaisford, D Rahman, S J Challacombe. 1993. Biodegradable microparticles for oral immunization.. https://doi.org/10.1016/0264-410x(93)90011-l

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