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An Alu sequence interrupts a human 5-hydroxytryptamine1D receptor pseudogene.

T Nguyen·A Marchese·J L Kennedy·A Petronis·S J Peroutka·P H Wu·B F O'Dowd
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Abstract

Molecular cloning studies have now identified six HTR genes encoding the biosynthesis of the structurally homologous human serotonin (5-hydroxytryptamine; 5-HT) receptors, namely 5-HTR1A, 5-HTR1B, 5-HTR1C, 5-HTR1D, 5-HTR2 and 5-HTRS31. Several of these receptors are encoded by intronless genes, and we now report the cloning of another intronless serotonergic HTR gene. This gene was cloned by a method using the polymerase chain reaction. The nucleotide sequence of this gene is most closely homologous to the 5-HTR1D gene; however, several stop codons, frame shifts and deletions are present in the coding region suggesting that this is a pseudogene which could not encode a functional receptor. Sequence analysis also revealed that the coding sequence of this pseudogene is disrupted by insertion of a 283-bp Alu repeat sequence.

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Amino Acid SequenceAnimalsBase SequenceBlotting, NorthernBlotting, SouthernCloning, MolecularHumansMolecular Sequence DataPan troglodytesPolymerase Chain ReactionPseudogenesRatsReceptors, SerotoninRepetitive Sequences, Nucleic AcidSequence Homology, Amino AcidSequence Homology, Nucleic AcidSpecies SpecificityJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, P.H.S.

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T Nguyen, A Marchese, J L Kennedy, A Petronis, S J Peroutka, P H Wu, B F O'Dowd. 1993-02-28. An Alu sequence interrupts a human 5-hydroxytryptamine1D receptor pseudogene.. https://doi.org/10.1016/0378-1119(93)90409-v

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