Sequence specificity of the core-binding factor.

I N Melnikova; B E Crute; S Wang; N A Speck

doi:10.1128/jvi.67.4.2408-2411.1993

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Sequence specificity of the core-binding factor.

I N Melnikova·B E Crute·S Wang·N A Speck

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Abstract

The core-binding factor (CBF) binds the conserved core motif in mammalian type C retrovirus enhancers. We analyzed the phosphate contacts made by CBF on the Moloney murine leukemia virus enhancer by ethylation interference assay. The phosphate contacts span 9 bp centered around the consensus core site. To examine the sequence preferences for CBF binding, we employed the technique of selected and amplified binding sequence footprinting (T. K. Blackwell and H. Weintraub, Science 250:1104-1110, 1990). The consensus binding site for CBF defined by selected and amplified binding sequence footprinting is PyGPyG GTPy.

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Animals Base Sequence Cattle Consensus Sequence DNA-Binding Proteins Enhancer Elements, Genetic Gene Expression Regulation, Viral In Vitro Techniques Molecular Sequence Data Moloney murine leukemia virus Nucleic Acid Conformation Phosphates Structure-Activity Relationship Transcription Factors Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

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I N Melnikova, B E Crute, S Wang, N A Speck. 1993. Sequence specificity of the core-binding factor.. https://doi.org/10.1128/jvi.67.4.2408-2411.1993

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