A subtractive hybridization method to isolate tissue-specific transcripts: application to the selection of new brain-specific products.

D Maréchal; C Forceille; D Breyer; D Delapierre; A Dresse

doi:10.1006/abio.1993.1055

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A subtractive hybridization method to isolate tissue-specific transcripts: application to the selection of new brain-specific products.

D Maréchal·C Forceille·D Breyer·D Delapierre·A Dresse

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Abstract

A method based on subtractive hybridization of brain complementary DNAs with peripheral messenger RNAs has enabled us to construct an enriched brain-specific cDNA library. Single-stranded cDNAs (ssc DNAs) were synthesized from brain polyadenylated mRNAs and subsequently hybridized with peripheral mRNAs immobilized on nitrocellulose membrane. Unhybridized sscDNAs were converted into double-stranded cDNAs and cloned into plasmid pUC13. The screening of the resulting library showed that a high percentage of the cloned cDNAs corresponded to mRNAs specifically transcribed in the brain.

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Animals Brain DNA Evaluation Studies as Topic Gene Library Male Nucleic Acid Hybridization Organ Specificity RNA, Messenger Rats Rats, Wistar Transcription, Genetic Journal Article

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D Maréchal, C Forceille, D Breyer, D Delapierre, A Dresse. 1993-02-01. A subtractive hybridization method to isolate tissue-specific transcripts: application to the selection of new brain-specific products.. https://doi.org/10.1006/abio.1993.1055

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