A simple intrapipette salt bridge.

S J Kleene

doi:10.1016/0165-0270(93)90136-f

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A simple intrapipette salt bridge.

S J Kleene

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Abstract

A simple method is described for achieving a salt bridge within a patch pipette. The tip of the pipette is filled with a Cl(-)-free solution that bathes the membrane patch. Above this is a Cl(-)-containing solution that surrounds the recording Ag/AgCl wire electrode. Addition of 0.07% (w/v) agarose to the solution in the tip prevents bulk mixing of the 2 phases within the pipette. This configuration makes it possible to remove Cl- from the membrane surface but maintain it at the electrode surface. The presence of the Cl(-)-free phase in the bottom of the patch pipette does not reduce the electrical stability of the recording electrode.

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Animals Chlorides Cilia Electrodes Electrophysiology Extracellular Space In Vitro Techniques Membrane Potentials Neurons, Afferent Rana pipiens Sepharose Silver Smell Solutions Journal Article Research Support, U.S. Gov't, P.H.S.

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S J Kleene. 1993. A simple intrapipette salt bridge.. https://doi.org/10.1016/0165-0270(93)90136-f

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