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Lambda kil-mediated lysis requires the phage context.

G R Reisinger·A Rietsch·W Lubitz·U Bläsi
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Abstract

The lambda kil gene has been shown to be responsible for premature lysis effected by addition of chloramphenicol between 15 and 20 min after thermal induction of a lambda prophage. Here, we localized the kil reading frame. The kil gene, represented by lambda orf47, overlaps genes cIII and gam. Expression of the plasmid-borne kil gene resulted in growth arrest, a reduction of colony-forming units and filament formation. However, kil-mediated cell lysis could not be triggered by chloramphenicol when the plasmid borne kil gene was expressed, suggesting that kil-induced cell lysis requires the phage context.

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Bacteriophage lambdaChloramphenicol O-AcetyltransferaseEscherichia coliGenes, ViralKineticsOpen Reading FramesPlasmidsRestriction MappingTime FactorsJournal ArticleResearch Support, Non-U.S. Gov't

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G R Reisinger, A Rietsch, W Lubitz, U Bläsi. 1993. Lambda kil-mediated lysis requires the phage context.. https://doi.org/10.1006/viro.1993.1222

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