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PubMed · 8460833

Dual microcolumn immunoaffinity liquid chromatography: an analytical application to human plasma proteins.

C L Flurer·M Novotny
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Abstract

Dual-column microcolumn immunoaffinity chromatography was accomplished by coupling immunoaffinity (IAC) and reversed-phase high-performance liquid chromatography (RP-HPLC) analysis columns. The IAC support was prepared by hydrophobically adsorbing the appropriate antibody onto an octyl silica-based stationary phase contained in a 250-microns-l.d. fused-silica microcolumn. Once the antigen was captured, the antigen-subtracted human plasma could be sent to the RP-HPLC microcolumn for plasma profiling. The antigen was quantitated by desorbing it from the IAC column and transferring it to the RP-HPLC system. Human plasma profiles were generated after removal of albumin, and transferrin and alpha 1-antitrypsin were quantitated with nanogram sensitivity. All analyses were accomplished with less than 1 microL of human plasma.

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AlbuminsAntigen-Antibody ComplexBlood ProteinsChromatography, AffinityChromatography, High Pressure LiquidHumansTransferrinalpha 1-AntitrypsinJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, P.H.S.

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C L Flurer, M Novotny. 1993-03-15. Dual microcolumn immunoaffinity liquid chromatography: an analytical application to human plasma proteins.. https://doi.org/10.1021/ac00054a028

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