Multiple column peptide synthesis, Part 2 (1, 2).

M Meldal; C B Holm; G Bojesen; M H Jakobsen; A Holm

doi:10.1111/j.1399-3011.1993.tb00333.x

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Multiple column peptide synthesis, Part 2 (1, 2).

M Meldal·C B Holm·G Bojesen·M H Jakobsen·A Holm

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Abstract

A manually operated apparatus for parallel multiple column soli-phase peptide synthesis is described. It employs Fmoc-amino acid-O-Dhbt or -Pfp esters in the continuous flow version of the polyamide method on small packed columns of kieselguhr supported resin in a reaction block of Teflon. The solvents and deprotecting reagents are dispensed from two washers in a parallel fashion and reagent consumption is low. Activated and protected amino acids are transferred from a dispenser tray as solutions, eight at a time. The use of the method is demonstrated by the synthesis of overlapping peptides from a protein structure and of analogous protease substrates. The products have been characterized by HPLC, FAB mass spectroscopy and amino acid analysis.

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Amino Acid Sequence Antigens, Bacterial Biochemistry Chromatography, High Pressure Liquid Endopeptidases Evaluation Studies as Topic Molecular Sequence Data Oligopeptides Resins, Plant Spectrometry, Mass, Fast Atom Bombardment Virulence Factors, Bordetella Journal Article Research Support, Non-U.S. Gov't

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M Meldal, C B Holm, G Bojesen, M H Jakobsen, A Holm. 1993. Multiple column peptide synthesis, Part 2 (1, 2).. https://doi.org/10.1111/j.1399-3011.1993.tb00333.x

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