[Artificial proteins with a given spatial structure and biological activity].

D A Dolgikh; A E Gabriélian; E V Navolotskaia; V V Chemeris; M P Kirpichnikov

PubMed · 8471646

[Artificial proteins with a given spatial structure and biological activity].

D A Dolgikh·A E Gabriélian·E V Navolotskaia·V V Chemeris·M P Kirpichnikov

Abstract

Biologically active fragment 131-138 of human interferon alpha 2 carrying blast-transforming activity of the protein was attached to the N-terminus of the de novo protein albebetin with predetermined tertiary structure by means of genetic engineering. The chimeric protein was expressed in a wheat germ cell-free translation system and tested for compactness, stability and biological activity. According to the tests used albebetin with interferon fragment has a compact and relatively stable structure. It binds murine thymocyte receptor with high affinity and activates efficiently thymocyte blast transformation at a concentration of 10(-11) M.

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Amino Acid Sequence Base Sequence Interferon-alpha Molecular Sequence Data Oligonucleotides Peptide Fragments Protein Structure, Tertiary Proteins Recombinant Fusion Proteins Thymus Gland English Abstract Journal Article

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D A Dolgikh, A E Gabriélian, E V Navolotskaia, V V Chemeris, M P Kirpichnikov. [Artificial proteins with a given spatial structure and biological activity].. https://pubmed.ncbi.nlm.nih.gov/8471646/

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