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Controlled release of polypeptides from polyanhydrides.

E Ron·T Turek·E Mathiowitz·M Chasin·M Hageman·R Langer
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Abstract

The effects of both polymer hydrophobicity and addition of stabilizers on the release and integrity of polymer-encapsulated proteins were studied. By using very hydrophobic poly[1,3-bis(p-carboxyhydroxy)hexane anhydride] with sucrose as an excipient, both recombinant bovine somatotropin and zinc insulin were released intact over 3 weeks. The released proteins appeared to maintain their integrity as judged by acidic reverse-phase HPLC, size-exclusion HPLC, radioimmunoassay, and conformation-sensitive immunoassays. Our results also suggest how polymer hydrophobicity can be used to enhance protein stability.

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AnimalsCattleDecanoic AcidsDelayed-Action PreparationsDrug CarriersGrowth HormoneImmunoassayIndicators and ReagentsInsulinKineticsPolyestersRecombinant ProteinsSpectrophotometry, InfraredTime FactorsJournal ArticleResearch Support, U.S. Gov't, P.H.S.

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E Ron, T Turek, E Mathiowitz, M Chasin, M Hageman, R Langer. 1993-05-01. Controlled release of polypeptides from polyanhydrides.. https://doi.org/10.1073/pnas.90.9.4176

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