Sequences and structures required for recombination between virus-associated RNAs.

P J Cascone; T F Haydar; A E Simon

doi:10.1126/science.8484119

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PubMed · 8484119

Sequences and structures required for recombination between virus-associated RNAs.

P J Cascone·T F Haydar·A E Simon

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Abstract

RNA recombination has been described for a number of viruses in the plant and animal kingdoms, but the mechanisms of selection of recombination sites are poorly understood. The nonrandom recombination between two subviral RNAs associated with turnip crinkle virus was used to study the requirement for specific sequences and structures in the generation of recombinant molecules. Single-base mutations that disrupted either the stem or the loop of one of the two computer-predicted stem-loop structures eliminated detectable recombinant molecules. However, recombinants were detected if compensatory mutations were generated that re-formed a stable hairpin structure. These results provide evidence for the necessity of specific structures in the formation of recombinant molecules in this system.

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Base Sequence Molecular Sequence Data Mutation Nucleic Acid Conformation Plant Viruses Plants RNA Viruses RNA, Viral Recombination, Genetic Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

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P J Cascone, T F Haydar, A E Simon. 1993-05-07. Sequences and structures required for recombination between virus-associated RNAs.. https://doi.org/10.1126/science.8484119

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