Polymorphic SSR (simple-sequence-repeat) markers for chromosome 20.

R Melis; P Bradley; T Elsner; M Robertson; E Lawrence; S Gerken; H Albertsen; R White

doi:10.1006/geno.1993.1140

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PubMed · 8486385

Polymorphic SSR (simple-sequence-repeat) markers for chromosome 20.

R Melis·P Bradley·T Elsner·M Robertson·E Lawrence·S Gerken·H Albertsen·R White

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Abstract

Chromosome 20-specific simple-sequence-repeat (SSR) markers were developed from a flow-sorted phage library (LL20NS01), subcloned in Bluescript, and screened with a tetranucleotide repeat, (AAAG)6, to identify potentially polymorphic loci. Of 100 clones sequenced, 39 were selected to construct primers. Of these 39, 22 were polymorphic. Reference to the CEPH linkage database (version 5) permitted genetic mapping of 16 of the new markers to specific regions of chromosome 20. Ten of the SSRs showed heterozygosity indices (above 70%) that would qualify them as potential index markers.

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Base Sequence Chromosome Mapping Chromosomes, Human, Pair 20 Cloning, Molecular DNA DNA Probes Female Genetic Markers Humans Male Molecular Sequence Data Pedigree Polymorphism, Genetic Recombination, Genetic Repetitive Sequences, Nucleic Acid Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

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R Melis, P Bradley, T Elsner, M Robertson, E Lawrence, S Gerken, H Albertsen, R White. 1993. Polymorphic SSR (simple-sequence-repeat) markers for chromosome 20.. https://doi.org/10.1006/geno.1993.1140

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