Three small ribooligonucleotides with specific arginine sites.

G J Connell; M Illangesekare; M Yarus

doi:10.1021/bi00072a002

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Three small ribooligonucleotides with specific arginine sites.

G J Connell·M Illangesekare·M Yarus

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Abstract

Arginine-binding RNA motifs are important to protein-RNA interaction and perhaps also for Archean biochemistry. Selection-amplification was used to isolate three RNAs that are eluted by free arginine from an L-arginine affinity column (Kd approximately 0.2-0.4 mM). The binding sites contain specific internal and bulge loops, whose sequences can include arginine coding triplets. Binding is highly specific for arginine, but all three motifs, like the self-splicing group I intron, also bind guanosine 5'-monophosphate. One site is stereoselective, somewhat preferring D-arginine.

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Arginine Base Sequence Binding Sites Kinetics Molecular Sequence Data Nucleic Acid Conformation Oligoribonucleotides Polymerase Chain Reaction Structure-Activity Relationship Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

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G J Connell, M Illangesekare, M Yarus. 1993-06-01. Three small ribooligonucleotides with specific arginine sites.. https://doi.org/10.1021/bi00072a002

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