Catalytic polyclonal antibodies.

D B Stephens; B L Iverson

doi:10.1006/bbrc.1993.1577

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Catalytic polyclonal antibodies.

D B Stephens·B L Iverson

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Abstract

The production and characterization of rabbit polyclonal antibodies that catalyze the hydrolysis of trityl protecting groups is described. The catalytic activity increased throughout the immunization regimen, exhibiting a later and steeper rise than the simple hapten-specific antibody titer. The catalytic polyclonal antibodies displayed saturation behavior, consistent with classic Michaelis-Menton kinetics. An apparent catalytic rate enhancement (kcat/kuncat) of 125 and a Km of 31 microM were measured for the polyclonal sample. Inhibition studies indicated that 12% of the antibodies either binds the hapten or is a catalyst. Analyzing polyclonal antibodies for catalytic activity is proposed as a substantially faster, more cost effective and more general way to screen new hapten designs for their relative abilities to produce antibodies with catalytic activity.

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Animals Antibodies Catalysis Haptens Immunoglobulins Indicators and Reagents Kinetics Rabbits Journal Article Research Support, Non-U.S. Gov't

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D B Stephens, B L Iverson. 1993-05-14. Catalytic polyclonal antibodies.. https://doi.org/10.1006/bbrc.1993.1577

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