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PubMed · 8509344

Phi X174 E complements lambda S and R dysfunction for host cell lysis.

W D Roof·R Young
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Abstract

Hybrid lambda phages which have the E lysis gene of the bacteriophage phi X174 in cis to defective nonsense and deletion alleles of the normal lambda lysis genes S and R have been constructed and shown to be fully competent for plaque-forming ability, which demonstrates that the single-gene, lysozyme-independent lysis system of phi X174 and related phages can serve the lytic function for large complex phages. These hybrid phages are unable to form plaques on a slyD host. Moreover, plaque morphology indicates that in E-mediated lysis the soluble lambda R endolysin can participate in lysis, indicating that the protein E-mediated lesions are not completely sealed off from the periplasm.

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Bacteriophage lambdaBacteriophage phi X 174EndopeptidasesGenes, ViralGenetic Complementation TestGlycosyltransferasesViral ProteinsViral Structural ProteinsVirus ReplicationJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, P.H.S.

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W D Roof, R Young. 1993. Phi X174 E complements lambda S and R dysfunction for host cell lysis.. https://doi.org/10.1128/jb.175.12.3909-3912.1993

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