Precise large deletions by the PCR-based overlap extension method.

S D Senanayake; D A Brian

doi:10.1007/bf02907467

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Precise large deletions by the PCR-based overlap extension method.

S D Senanayake·D A Brian

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Abstract

The authors describe an efficient method for generating large deletions (> 200 nts) of precise length using the PCR-based method of gene splicing by overlap extension (1). This method is technically simple and less time consuming than conventional loop-out mutagenesis techniques requiring preparation of a single-stranded DNA template.

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Base Sequence Cloning, Molecular Coronavirus DNA Primers DNA, Recombinant Molecular Sequence Data Mutagenesis, Site-Directed Polymerase Chain Reaction RNA RNA, Viral Sequence Deletion Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

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S D Senanayake, D A Brian. 1995. Precise large deletions by the PCR-based overlap extension method.. https://doi.org/10.1007/bf02907467

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