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PubMed · 8521572

Delayed rectifier channels in human ventricular myocytes.

M W Veldkamp·A C van Ginneken·T Opthof·L N Bouman
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Abstract

BACKGROUND: Previous studies have shown that in heart there are two kinetically distinct components of delayed rectifier current: a rapidly activating component (IKr) and a more slowly activating component (IKs). The presence of IKr and/or IKs appears to be species dependent. We studied the nature of the delayed rectifier current in human ventricle in whole-cell and single-channel experiments. METHODS AND RESULTS: Ventricular myocytes were obtained from hearts of patients with ischemic or dilated cardiomyopathy. Single-channel currents and whole-cell tail currents were recorded at negative potentials directly after return from a depolarizing step. Single-channel currents were measured in the cell-attached patch configuration with 140 mmol/L K+ in the pipette. In the present study, we identified a voltage-dependent channel with a single-channel conductance of 12.9 +/- 0.8 pS (mean +/- SEM, n = 5) and a reversal potential near to the K+ equilibrium potential, suggesting that the channel is selective to K+ ions. Channel activity was observed only after a depolarizing step and increased with the duration and amplitude of the depolarization, indicating time- and voltage-dependent activation. Activation at +30 mV was complete within 300 milliseconds, and the time constant of activation, determined in the whole-cell configuration, was 101 +/- 25 milliseconds (mean +/- SEM, n = 4). The voltage dependence of activation could be described by a Boltzmann equation with a half-activation potential of -29.9 mV and a slope factor of 9.5 mV. The addition of the class III antiarrhythmic drug E-4031 completely blocked channel activity in one patch. No indications for the presence of IKs were found in these experiments. CONCLUSIONS: The conformity between the properties of IKr and those of the K+ channel in the present study strongly suggests that IKr is present in human ventricle.

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Action PotentialsAnti-Arrhythmia AgentsCardiomyopathy, DilatedCells, CulturedHeart VentriclesHumansMyocardial IschemiaMyocardiumPiperidinesPotassium ChannelsPyridinesJournal Article

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BibTeXRIS

M W Veldkamp, A C van Ginneken, T Opthof, L N Bouman. 1995-12-15. Delayed rectifier channels in human ventricular myocytes.. https://doi.org/10.1161/01.cir.92.12.3497

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