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PubMed · 8528139

Functional domain mapping using M13 deletions.

N Grammatikakis·B P Toole
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Abstract

We describe a convenient approach for concomitant functional characterization of peptide domains (monoclonal antibody epitopes, receptor-ligand, DNA-protein, and protein-protein interaction sites, etc) encoded by the sequential series of overlapping M13 subclones generated for nucleotide sequence determination of a new cDNA. We have employed this method to rapidly map the location and amino acid sequence of an epitope-containing domain within a polypeptide encoded by a newly isolated cDNA.

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Amino Acid SequenceAntibodies, MonoclonalBacteriophage M13Blotting, WesternCloning, MolecularDNA, ComplementaryElectrophoresis, Agar GelEpitopesGenes, ViralMolecular Sequence DataMutagenesisPeptide BiosynthesisPeptidesProtein BiosynthesisProteinsRecombinant ProteinsSequence Deletionbeta-GalactosidaseJournal ArticleResearch Support, U.S. Gov't, P.H.S.

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N Grammatikakis, B P Toole. 1995. Functional domain mapping using M13 deletions.. https://pubmed.ncbi.nlm.nih.gov/8528139/

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