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DNA separation with field inversion capillary electrophoresis.

C Heller·C Pakleza·J L Viovy
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Abstract

We have built an automated pulsed field capillary electrophoresis system on the basis of a commercially available device. Using entangled polymer solutions as separating matrix, we demonstrate a considerable improvement of separation of double-stranded DNA in the range of 1-50 kbp under pulsed field conditions. The influence of the main parameters, i.e. pulse frequency and electric field, is studied and the results are compared to existing electrophoresis theories.

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AutoanalysisBacteriophage T4Bacteriophage lambdaBacteriophage phi X 174DNA, ViralElectricityElectrophoresisElectrophoresis, CapillaryModels, ChemicalPolymersSolutionsTime FactorsComparative StudyJournal ArticleResearch Support, Non-U.S. Gov't

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C Heller, C Pakleza, J L Viovy. 1995. DNA separation with field inversion capillary electrophoresis.. https://doi.org/10.1002/elps.11501601235

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