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A simple method for detection of IgG rheumatoid factor.

C M Riera·A Risemberg·A Strusberg·C Yantorno
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Abstract

A simple method for detection of IgG rheumatoid factor (RF) in sera and joint fluid is described. The technique is based on the action of 0.1 M 2-mercaptoethanol mixed directly with the material. After 2 h of incubation determinations of human anti-IgG were performed by latex agglutination test. Comparison with data obtained by using the conventional method, sequential 24 h treatment with 0.1 M 2-mercaptoethanol and 0.01 M iodoacetamide, shows similar results for both methods. A correlation was observed between the presence of IgGRF in synovial fluid and a severe clinical course with invalidating forms in patients suffering rheumatoid arthritis.

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Antigen-Antibody ComplexArthritis, RheumatoidAutoantibodiesFemaleHumansImmunoglobulin GLatex Fixation TestsMaleMercaptoethanolRheumatoid FactorSynovial FluidJournal Article

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C M Riera, A Risemberg, A Strusberg, C Yantorno. 1977. A simple method for detection of IgG rheumatoid factor.. https://doi.org/10.1016/0022-1759(77)90059-x

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