GUF1, a gene encoding a novel evolutionarily conserved GTPase in budding yeast.

G L Kiser; T A Weinert

doi:10.1002/yea.320111312

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GUF1, a gene encoding a novel evolutionarily conserved GTPase in budding yeast.

G L Kiser·T A Weinert

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Abstract

While sequencing a region of chromosome IV adjacent to the checkpoint gene MEC3, we identified a gene we call GUF1 (GTPase of Unknown Function), which predicts a 586 amino acid GTPase of the elongation factor-type class. The predicted Guf1p protein bears striking sequence similarity to both LepA from Escherichia coli (43% identical) and LK1236.1 from Caenorhabditis elegans (42% identical). Analysis of both a guf1 delta deletion and a putative constitutive-activating mutant (GUF1HG) revealed that GUF1 is not essential nor did mutant cells reveal any marked phenotype.

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Amino Acid Sequence Base Sequence Biological Evolution Conserved Sequence GTP Phosphohydrolases Genes, Fungal Molecular Sequence Data Saccharomyces cerevisiae Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

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G L Kiser, T A Weinert. 1995. GUF1, a gene encoding a novel evolutionarily conserved GTPase in budding yeast.. https://doi.org/10.1002/yea.320111312

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