Fine structural observations on the human sperm nuclear matrix.

E C Chew; S B Cheng-Chew; H F Yam; L Yang; P Y Leung; C T Liew; M X Ding

PubMed · 8562881

Fine structural observations on the human sperm nuclear matrix.

E C Chew·S B Cheng-Chew·H F Yam·L Yang·P Y Leung·C T Liew·M X Ding

Abstract

The nucleus of the mammalian sperm is formed after a series of morphological and biochemical changes during spermatogenesis. The human sperm nucleus, after sequential extraction with detergents, nuclease and ammonium sulfate, consists of a fibroskeletal structure which maintains the original nuclear shape. The chromatin-depleted skeleton is formed by thick and thin fibers as well as electron-dense patches of different sizes. These highly branched matrix fibers had average diameters of 35 and 12 nm. Polarization of the fibroskeletal structure is apparent and can be used as a good model to study the function of nuclear matrix in nuclear compartmentation in germ cells.

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Chromatin Cytoskeleton Humans Male Microscopy, Electron Nuclear Matrix Sperm Head Journal Article Research Support, Non-U.S. Gov't

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BibTeX RIS

E C Chew, S B Cheng-Chew, H F Yam, L Yang, P Y Leung, C T Liew, M X Ding. Fine structural observations on the human sperm nuclear matrix.. https://pubmed.ncbi.nlm.nih.gov/8562881/

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