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PubMed · 8563017

Coaxial continuous-flow fast-atom bombardment vs electrospray ionization: a sensitivity comparison on a magnetic sector instrument.

L J Deterding·M Khaledi·K B Tomer
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Abstract

The performance of an electrospray (ESI) source was evaluated and compared to that of a coaxial continuous-flow fast-atom bombardment source on a magnetic mass spectrometer using ten different peptides over the mass range of 400 to 3500 Da and using a variety of scanning modes. Results show that sensitivities using the two ionization techniques are similar, with limits-of-detection in the attomole to low femtomole range. In addition, proteins can be routinely detected using ESI at femtomole levels. The observance of the noncovalent complex of RNase A and cytidine 2'-monophosphate yields evidence that these complexes can be studied using instruments that operate at high accelerating voltages.

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Cytidine MonophosphateEvaluation Studies as TopicPeptidesRibonucleasesSpectrometry, Mass, Fast Atom BombardmentComparative StudyJournal Article

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BibTeXRIS

L J Deterding, M Khaledi, K B Tomer. 1996. Coaxial continuous-flow fast-atom bombardment vs electrospray ionization: a sensitivity comparison on a magnetic sector instrument.. https://doi.org/10.1002/(sici)1097-0231(19960115)10%3A1%3C60%3A%3Aaid-rcm446%3E3.0.co%3B2-3

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PubMed2001

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