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Separation of gentamicin C-complex into five components by Craig distribution.

K M Byrne·A S Kershner·H Maehr·J A Marquez·C P Schaffner
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Abstract

A gentamicin C-complex preparation was separated preparatively into five components by Craig distribution. Gentamicins C1, C2, and C1a were the major components, whereas components C2a and C2b represented only 4% of the total C-complex mixture. Paper chromatographic analysis showed that the gentamicin C2b separated by Craig distribution was identical with a gentamicin isolated from Micromonospora purpurea var. JI-33 fermentation broth and identified as 6'-N-methylgentamicin C1a. Similarly, component C2a was identical with a previously separated gentamicin component tentatively identified as the 6'-C epimer of gentamicin C2.

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Biological AssayChemical PhenomenaChemistryChromatography, PaperChromatography, Thin LayerDiffusionGentamicinsMathematicsMethodsStaphylococcus aureusJournal Article

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K M Byrne, A S Kershner, H Maehr, J A Marquez, C P Schaffner. 1977-01-21. Separation of gentamicin C-complex into five components by Craig distribution.. https://doi.org/10.1016/s0021-9673(00)80933-4

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