DAPI: a DNA-specific fluorescent probe.

J Kapuscinski

doi:10.3109/10520299509108199

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DAPI: a DNA-specific fluorescent probe.

J Kapuscinski

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Abstract

DAPI (4',6-diamidino-2-phenylindole) is a DNA-specific probe which forms a fluorescent complex by attaching in the minor grove of A-T rich sequences of DNA. It also forms nonfluorescent intercalative complexes with double-stranded nucleic acids. The physicochemical properties of the dye and its complexes with nucleic acids and history of the development of this dye as a biological stain are described. The application of DAPI as a DNA-specific probe for flow cytometry, chromosome staining, DNA visualization and quantitation in histochemistry and biochemistry is reviewed. The mechanisms of DAPI-nucleic acid complex formation including minor groove binding, intercalation and condensation are discussed.

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Animals Base Sequence Chemical Phenomena Chemistry, Physical DNA Fluorescent Dyes Humans Indoles Molecular Sequence Data Sensitivity and Specificity Journal Article Research Support, Non-U.S. Gov't Review

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J Kapuscinski. 1995. DAPI: a DNA-specific fluorescent probe.. https://doi.org/10.3109/10520299509108199

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