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Anisakis simplex: stage-specific antigens recognized by mice.

R Iglesias·J Leiro·F M Ubeira·M T Santamarina·M L Sanmartín
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Abstract

To investigate antigenic differences between the developmental stages of Anisakis simplex, somatic, excretion-secretion and detergent-solubilized surface antigen preparations of third- and fourth-stage A. simplex larvae, and a somatic antigen preparation of adults, were characterized by SDS-PAGE and immunoblotting using mouse immune serum containing antibodies against the two larval stages. Excretion-secretion and surface antigen preparations from third-stage larvae behaved very differently from those of fourth-stage larvae, in both SDS-PAGE and immunoblotting; this suggests that certain excretion-secretion and surface antigens are highly stage-specific. By contrast, somatic components of third- and fourth-stage larvae were found to have very similar banding patterns, suggesting conservation of these components during the development of the parasite in mice. The SDS-PAGE and immunoblotting results for adult somatic components seem to support this hypothesis.

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AnimalsAnisakiasisAnisakisAntibodies, HelminthAntibody SpecificityAntigens, HelminthElectrophoresis, Polyacrylamide GelImmunoblottingLarvaMaleMiceMice, Inbred BALB CMice, Inbred CBAComparative StudyJournal ArticleResearch Support, Non-U.S. Gov't

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R Iglesias, J Leiro, F M Ubeira, M T Santamarina, M L Sanmartín. 1995. Anisakis simplex: stage-specific antigens recognized by mice.. https://doi.org/10.1017/s0022149x00014899

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