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Development and validation of a solid-phase extraction and high-performance liquid chromatographic assay for a novel fluorinated 2-nitroimidazole hypoxia probe (SR-4554) in Balb/c mouse plasma.

E O Aboagye·M A Graham·A D Lewis·P Workman·A B Kelson·M Tracy
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Abstract

N-(2-Hydroxy-3,3,3-trifluoropropyl)-2-(2-nitro-1-imidazolyl) acetamide, a novel 2-nitroimidazole, is currently being developed as a non-invasive probe for tumour hypoxia. A sensitive (minimum quantifiable level = 25 ng/ml; C.V. = 6.01%) and selective assay has, therefore, been developed for the analysis of this compound in mouse plasma. The assay employed a solid-phase extraction followed by a rapid (10 min) HPLC analysis with UV-photodiode-array detection. No drug-related metabolites were observed in plasma when mice were treated with 180 mg/kg of the drug. The assay has proved to be suitable for studying the plasma pharmacokinetics of this fluorinated 2-nitroimidazole in mice.

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AnimalsCalibrationCell HypoxiaChromatography, High Pressure LiquidFemaleIndicators and ReagentsMass SpectrometryMiceMice, Inbred BALB CMolecular ProbesNitroimidazolesQuality ControlReproducibility of ResultsSensitivity and SpecificityJournal ArticleResearch Support, Non-U.S. Gov't

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E O Aboagye, M A Graham, A D Lewis, P Workman, A B Kelson, M Tracy. 1995-10-06. Development and validation of a solid-phase extraction and high-performance liquid chromatographic assay for a novel fluorinated 2-nitroimidazole hypoxia probe (SR-4554) in Balb/c mouse plasma.. https://doi.org/10.1016/0378-4347(95)00204-v

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