Acetylcholine release from dissociated striatal cells.

I S Login; K Borland; M B Harrison; M E Ragozzino; P E Gold

doi:10.1016/0006-8993(95)00996-4

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Acetylcholine release from dissociated striatal cells.

I S Login·K Borland·M B Harrison·M E Ragozzino·P E Gold

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Abstract

To study the regulation of striatal acetylcholine (ACH) release, adult male rat striata were dissociated and incubated with 3H-choline to synthesize 3H-ACH. Fractional 3H-ACH efflux per min during continuous perifusion was: (1) tightly regulated; (2) dependent on calcium influx; (3) stimulated by 10 mM K+ and 1 mM glutamate; and (4) comparable to ACH release detected by HPLC. Thus, acutely dissociated striata exhibit calcium-sensitive, voltage-dependent secretion of 3H-ACH and direct receptor-mediated stimulation of release through the glutamate receptor family. This new approach toward cholinergic secretory physiology will help clarify complex striatal circuitry.

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Acetylcholine Animals Calcium Chromatography, High Pressure Liquid Corpus Striatum Glutamic Acid Male Potassium Rats Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

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I S Login, K Borland, M B Harrison, M E Ragozzino, P E Gold. 1995-10-30. Acetylcholine release from dissociated striatal cells.. https://doi.org/10.1016/0006-8993(95)00996-4

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