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PubMed · 8594299

A Gi2alpha antisense oligonucleotide differentiates morphine antinociception, constipation and acute dependence in mice.

R B Raffa·T L Goode·R P Martinez·H I Jacoby
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Abstract

In the same mice in which the intracerebroventricular (i.c.v.) administration of antisense oligodeoxyribonucleotide (oligo) directed against the Gi2alpha (but not Gi1alpha, Gi3alpha or G(s)alpha) G-protein subunits attenuated i.c.v. morphine-induced antinociception in the tail-flick test, none of the oligos altered naloxone-precipitated jumping (acute dependence). Likewise, none of the oligos significantly altered morphine-induced constipation. Hence, i.c.v. morphine-induced antinociception might be preferentially mediated via transduction pathway(s) different from constipation or acute dependence, offering novel opportunities for drug discovery.

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Analgesics, OpioidAnimalsBase SequenceCerebral VentriclesConstipationGTP-Binding ProteinsInjections, IntraventricularKineticsMaleMiceMice, Inbred StrainsMolecular Sequence DataMorphineMorphine DependenceNaloxoneOligonucleotides, AntisensePainReaction TimeJournal Article

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R B Raffa, T L Goode, R P Martinez, H I Jacoby. 1996. A Gi2alpha antisense oligonucleotide differentiates morphine antinociception, constipation and acute dependence in mice.. https://doi.org/10.1016/0024-3205(95)02300-3

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