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PubMed · 8595721

Highly sensitive immunoradiometric assay for serum thyroglobulin with minimal interference from autoantibodies.

P Y Marquet·A Daver·R Sapin·B Bridgi·J P Muratet·D J Hartmann·F Paolucci·B Pau
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Abstract

Five monoclonal antibodies (MAbs) directed against antigenic domains on thyroglobulin (Tg) not recognized by most anti-Tg human autoantibodies (aAbs) have been used to develop an improved IRMA for serum Tg with a limit of detection of 0.2 micrograms/L. Samples are incubated for 3 h in tubes coated with four anti-Tg MAbs. After washing, the tubes are incubated with the tracer MAb for 20 h at room temperature. Dilution and reproducibility tests demonstrated assay reliability. Tests performed on samples with (n = 361) or without (n = 283) aAbs showed that the TG IRMA Pasteur is largely independent of the marked interference generally caused by aAbs. These results were confirmed with an extended population of 2759 samples. For a cutoff of 1 micrograms/L, sensitivity and specificity were 0.97 and 1, respectively, in a follow-up of differentiated thyroid carcinoma in patients treated by total thyroidectomy.

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AnimalsAntibodies, MonoclonalAutoantibodiesHumansImmunoradiometric AssayMiceNeoplasm MetastasisROC CurveReagent Kits, DiagnosticReproducibility of ResultsSensitivity and SpecificityThyroglobulinThyroid NeoplasmsThyroidectomyComparative StudyJournal Article

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P Y Marquet, A Daver, R Sapin, B Bridgi, J P Muratet, D J Hartmann, F Paolucci, B Pau. 1996. Highly sensitive immunoradiometric assay for serum thyroglobulin with minimal interference from autoantibodies.. https://pubmed.ncbi.nlm.nih.gov/8595721/

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