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Locus control region function and heterochromatin-induced position effect variegation.

R Festenstein·M Tolaini·P Corbella·C Mamalaki·J Parrington·M Fox·A Miliou·M Jones·D Kioussis
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Abstract

Human CD2 locus control region (LCR) sequences are shown here to be essential for establishing an open chromatin configuration. Transgenic mice carrying an hCD2 mini-gene attached only to the 3' CD2 transcriptional enhancer exhibited variegated expression when the transgene integrated in the centromere. In contrast, mice carrying a transgene with additional 3' sequences showed no variegation even when the latter integrated in centromeric positions. This result suggests that LCRs operate by ensuring an open chromatin configuration and that a short region, with no enhancer activity, functions in the establishment, maintenance, or both of an open chromatin domain.

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AnimalsCD2 AntigensCentromereEnhancer Elements, GeneticGene Expression RegulationHeterochromatinHumansIn Situ Hybridization, FluorescenceMiceMice, TransgenicRegulatory Sequences, Nucleic AcidT-LymphocytesTransgenesJournal ArticleResearch Support, Non-U.S. Gov't

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R Festenstein, M Tolaini, P Corbella, C Mamalaki, J Parrington, M Fox, A Miliou, M Jones, D Kioussis. 1996-02-23. Locus control region function and heterochromatin-induced position effect variegation.. https://doi.org/10.1126/science.271.5252.1123

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