Streptozotocin, an inducer of NAD+ decrease, attenuates M-potassium current inhibition by ATP, bradykinin, angiotensin II, endothelin 1 and acetylcholine in NG108-15 cells.

H Higashida; A Egorova; N Hoshi; M Noda

doi:10.1016/0014-5793(95)01516-7

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PubMed · 8603696

Streptozotocin, an inducer of NAD+ decrease, attenuates M-potassium current inhibition by ATP, bradykinin, angiotensin II, endothelin 1 and acetylcholine in NG108-15 cells.

H Higashida·A Egorova·N Hoshi·M Noda

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Abstract

The M-potassium current was inhibited by bath application of 100 micron ATP, 10 nM bradykinin, 100 nM angiotensin II and 100 nM endothelin 1 as well as by 10 micron acetylcholine in an m1-muscarinic acetylcholine receptor-transformed NG108-15 cell line. The inhibition of M-current was attenuated in cells pretreated with 5 mM streptozotocin for 5-15 h and restored by simultaneous incubation with 5 mM nicotinamide. The results suggest that signal transduction from these five different receptors to M channels shares a common pathway which is susceptible to a streptozotocin-induced decrease in cellular NAD+ content.

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H Higashida, A Egorova, N Hoshi, M Noda. 1996-02-05. Streptozotocin, an inducer of NAD+ decrease, attenuates M-potassium current inhibition by ATP, bradykinin, angiotensin II, endothelin 1 and acetylcholine in NG108-15 cells.. https://doi.org/10.1016/0014-5793(95)01516-7

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