A second double-stranded RNA virus from yeast.

C M Park; J D Lopinski; J Masuda; T H Tzeng; J A Bruenn

doi:10.1006/viro.1996.0083

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A second double-stranded RNA virus from yeast.

C M Park·J D Lopinski·J Masuda·T H Tzeng·J A Bruenn

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Abstract

Two double-stranded RNA viruses exist as permanent persistent infections of the yeast Saccharomyces cerevisiae: ScVL1 and ScVLa. Both belong to the Totiviridae, which include a number of fungal and protozoan double-stranded RNA viruses. Although ScVL1 and ScVLa share the same genomic organization and mode of expression and coexist in the same cells, they show no evidence of recombination: with one limited exception, sequence conservation is detectable only in regions conserved in all totiviruses. Both have two open reading frames on their single essential RNAs: cap (encoding a capsid polypeptide) and pol (encoding an RNA-dependent RNA polymerase). The ScVLa virus, like ScVL1, appears to express its Pol domain by a -1 translational frameshift.

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Amino Acid Sequence Base Sequence Capsid DNA, Viral Genome, Viral Molecular Sequence Data Open Reading Frames RNA Viruses RNA, Double-Stranded Saccharomyces cerevisiae Journal Article Research Support, U.S. Gov't, Non-P.H.S.Research Support, U.S. Gov't, P.H.S.

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C M Park, J D Lopinski, J Masuda, T H Tzeng, J A Bruenn. 1996-02-15. A second double-stranded RNA virus from yeast.. https://doi.org/10.1006/viro.1996.0083

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