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PubMed · 8622874

Further characterization of the c-mos transcript and its cell cycle specific expression in NIH3T3 cells.

C Gao·R B Arlinghaus·B Singh
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Abstract

The mouse c-mos proto-oncogene is primarily expressed in germ cells. Our previous studies demonstrated c-mos RNA expression in mouse somatic cells, with the highest level present in the G2 phase of the cell cycle (Tsui et al., 1993). We have identified the transcription start site of this G2 specific c-mos transcript to be located about 1580 bp upstream from the open reading frame based on RT-PCR and RNase protection experiments. Upstream sequences containing this transcription start site directed highest expression of the luciferase reporter gene in M phase of the cell cycle. These results suggest that c-mos transcripts are produced in G2 phase and that c-Mos protein albeit at extremely low levels would accumulate in M phase.

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3T3 CellsAnimalsBase SequenceCell CycleDNA ProbesLuciferasesMiceMolecular Sequence DataProto-Oncogene Proteins c-mosRNA, MessengerTranscription, GeneticJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, P.H.S.

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C Gao, R B Arlinghaus, B Singh. 1996-04-04. Further characterization of the c-mos transcript and its cell cycle specific expression in NIH3T3 cells.. https://pubmed.ncbi.nlm.nih.gov/8622874/

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