Improved method for selecting RNA-binding activities in vivo.

D E Fouts; D W Celander

doi:10.1093/nar/24.8.1582

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PubMed · 8628698

Improved method for selecting RNA-binding activities in vivo.

D E Fouts·D W Celander

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Abstract

RNA challenge phages are modified versions of bacteriophage P22 that allow one to select directly for a specific RNA-protein interaction in vivo. The original construction method for generating a bacteriophage that encodes a specific RNA target requires two homologous recombination reactions between plasmids and phages in bacteria. An improved method is described that enables one to readily construct RNA challenge phages through a single homologous recombination reaction in vivo. We have applied the new method to construct a derivative of P22R17, an RNA challenge phage that undergoes lysogenic development in bacterial cells that express the bacteriophage R17/MS2 coat protein.

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Bacteriophage P22 Base Sequence Binding Sites Molecular Sequence Data Nucleic Acid Conformation RNA, Viral RNA-Binding Proteins Recombination, Genetic Journal Article Research Support, U.S. Gov't, P.H.S.

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D E Fouts, D W Celander. 1996-04-15. Improved method for selecting RNA-binding activities in vivo.. https://doi.org/10.1093/nar%2F24.8.1582

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