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Karyotyping human chromosomes by combinatorial multi-fluor FISH.

M R Speicher·S Gwyn Ballard·D C Ward
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Abstract

We have developed epifluorescence filter sets and computer software for the detection and discrimination of 27 different DNA probes hybridized simultaneously. For karyotype analysis, a pool of human chromosome painting probes, each labelled with a different fluor combination, was hybridized to metaphase chromosomes prepared from normal cells, clinical specimens, and neoplastic cell lines. Both simple and complex chromosomal rearrangements could be detected rapidly and unequivocally; many of the more complex chromosomal abnormalities could not be delineated by conventional cytogenetic banding techniques. Our data suggest that multiplex-fluorescence in situ hybridization (M-FISH) could have wide clinical utility and complement standard cytogenetics, particularly for the characterization of complex karyotypes.

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Chromosome AberrationsChromosomes, Artificial, YeastChromosomes, HumanDNA ProbesFemaleFluorescent DyesGene RearrangementHumansImage Processing, Computer-AssistedIn Situ Hybridization, FluorescenceKaryotypingMaleSoftwareTumor Cells, CulturedJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, Non-P.H.S.

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M R Speicher, S Gwyn Ballard, D C Ward. 1996. Karyotyping human chromosomes by combinatorial multi-fluor FISH.. https://doi.org/10.1038/ng0496-368

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