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PubMed · 8633074

Mutation detection with MutH, MutL, and MutS mismatch repair proteins.

J Smith·P Modrich
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Abstract

Escherichia coli methyl-directed mismatch repair is initiated by MutS-, MutL-, and ATP-dependent activation of MutH endonuclease, which cleaves at d(GATC) sites in the vicinity of a mismatch. This reaction provides an efficient method for detection of mismatches in heteroduplexes produced by hybridization of genetically distinct sequences after PCR amplification. Multiple examples of transition and transversion mutations, as well as one, two, and three nucleotide insertion/deletion mutants, have been detected in PCR heteroduplexes ranging in size from 400 bp to 2.5 kb. Background cleavage of homoduplexes is largely due to polymerase errors that occur during amplification, and the MutHLS reaction provides an estimate of the incidence of mutant sequences that arise during PCR.

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Adenosine TriphosphatasesBacterial ProteinsBase SequenceDNA Mutational AnalysisDNA RepairDNA Repair EnzymesDNA, BacterialDNA-Binding ProteinsDeoxyguanine NucleotidesEndodeoxyribonucleasesEscherichia coliEscherichia coli ProteinsGenes, BacterialKineticsMethylationModels, GeneticMutL ProteinsMutS DNA Mismatch-Binding ProteinNucleic Acid HeteroduplexesPlasmidsPolymerase Chain ReactionSubstrate SpecificityTemplates, GeneticJournal ArticleResearch Support, U.S. Gov't, P.H.S.

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J Smith, P Modrich. 1996-04-30. Mutation detection with MutH, MutL, and MutS mismatch repair proteins.. https://doi.org/10.1073/pnas.93.9.4374

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