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PubMed · 8643503

DNA analysis and diagnostics on oligonucleotide microchips.

G Yershov·V Barsky·A Belgovskiy·E Kirillov·E Kreindlin·I Ivanov·S Parinov·D Guschin·A Drobishev·S Dubiley·A Mirzabekov
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Abstract

We present a further development in the technology of sequencing by hybridization to oligonucleotide microchips (SHOM) and its application to diagnostics for genetic diseases. A robot has been constructed to manufacture sequencing "microchips." The microchip is an array of oligonucleotides immobilized into gel elements fixed on a glass plate. Hybridization of the microchip with fluorescently labeled DNA was monitored in real time simultaneously for all microchip elements with a two-wavelength fluorescent microscope equipped with a charge-coupled device camera. SHOM has been used to detect beta-thalassemia mutations in patients by hybridizing PCR-amplified DNA with the microchips. A contiguous stacking hybridization technique has been applied for the detection of mutations; it can simplify medical diagnostics and enhance its reliability. The use of multicolor monitoring of contiguous stacking hybridization is suggested for large-scale diagnostics and gene polymorphism studies. Other applications of the SHOM technology are discussed.

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Base SequenceBiotechnologyDNADNA Mutational AnalysisDNA PrimersEvaluation Studies as TopicGenetic Diseases, InbornGenetic TechniquesGlobinsHumansMolecular Sequence DataNucleic Acid HybridizationOligonucleotidesPolymerase Chain ReactionPolymorphism, GeneticRoboticsbeta-ThalassemiaJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, Non-P.H.S.

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G Yershov, V Barsky, A Belgovskiy, E Kirillov, E Kreindlin, I Ivanov, S Parinov, D Guschin, A Drobishev, S Dubiley, A Mirzabekov. 1996-05-14. DNA analysis and diagnostics on oligonucleotide microchips.. https://doi.org/10.1073/pnas.93.10.4913

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