Characterization of murine intercellular adhesion molecule-2.

H Xu; J K Bickford; E Luther; C Carpenito; F Takei; T A Springer

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PubMed · 8648141

Characterization of murine intercellular adhesion molecule-2.

H Xu·J K Bickford·E Luther·C Carpenito·F Takei·T A Springer

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Abstract

Rat mAbs were raised against murine intercellular adhesion molecule-2 (ICAM-2). Immune precipitation and purification reveal that the murine ICAM-2 glycoprotein is 55 kDa and is similar in size to human ICAM-2. ICAM-2 is expressed on a variety of leukocyte cell lines, including T and B lymphoma, mastocytoma, and macrophage lines. ICAM-2 is well expressed on endothelioma cell lines, and in contrast to ICAM-1, expression is not increased by inflammatory cytokines. One of the mAb to ICAM-2 partially or completely inhibits binding of cells expressing LFA-1 to purified ICAM-2, and binding of cells expressing ICAM-2 to purified LFA-1. The findings in the mouse are congruent with those in the human, suggesting functional conservation of ICAM-2 across species.

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Animals Antibodies, Monoclonal Antigens, CD Cell Adhesion Cell Adhesion Molecules Cell Line Cloning, Molecular Humans Lymphocyte Function-Associated Antigen-1 Mice Protein Binding Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

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H Xu, J K Bickford, E Luther, C Carpenito, F Takei, T A Springer. 1996-06-15. Characterization of murine intercellular adhesion molecule-2.. https://pubmed.ncbi.nlm.nih.gov/8648141/

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